Techne PrimeQ manuals

Techne PrimeQ User Manual (181 pages)

Brand: Techne | Category: Equipment | Size: 23.62 MB |

English

Table of contents

OPERATOR’S MANUAL

1

How to use this guide

3

Contents

7

1 Introduction

13

1.1 PrimeQ

15

1.2 Quansoft

15

1.2.3 Program Editor

16

1.2.4 PrimeQ reports

16

1.2.5 Data Analysis

16

1.3 Unpacking

17

1.4 Warning!

17

1.5 Uses of PrimeQ

17

1.6 Plates

17

1.7 Thermal seal

18

1.8 Contacting us

18

1.9 Installing the hardware

18

1.9.3 Operator safety

20

1.9.4 Installation

20

1.10 Before switching on

23

1.11 Technical Specification

24

1.12 Working conditions

25

1.13 Guarantee

25

WARRANTY

26

IF BROKEN

26

1.14.4 Software upgrades

29

1.15.1 Function keys

30

1.15.2 Power up screen

30

1.15.3 Beeper

30

1.15.4 Before you start

31

1.15.5 Start-up procedure

31

1.16.8 Points to remember

35

1.18 LED power settings

37

1.19 Inserting a plate

37

1.20 After use

38

2 Getting started

39

2.1 Introduction to PrimeQ

41

2.2.1 PCR

42

2.3.1 Intercalating dyes

44

2.3.3 Molecular Beacons

45

2.4 System overview

46

2.5 Optics module

46

2.6 Thermal cycling module

47

2.7 Scanning module

48

3 Quansoft

49

3.1 Introducing Quansoft

51

3.2 Software overview

52

3.2.1 Home page

53

3.2.2 The Editors

53

3.2.2.3 Program Editor

54

3.2.2.4 Results Editor

54

3.3 Using Quansoft

55

3.3.2 Main screen

56

3.3.3 Navigation bar

56

3.3.4 Title bar functions

57

3.3.5 Menu bar functions

58

3.3.6 Accessing the editors

59

3.4.1 An Overview

60

3.4.3 Setting up a program

62

Comment [MO1]:

65

Discussed between

65

Matt and Christian

65

3.5 Running an experiment

86

3.5.2 Monitoring the run

89

3.6 LED intensity settings

92

3.7 Results Editor

93

3.7.3 Editing a graph

96

3.7.5 Log/Audit trail

99

3.7.6 Report

99

3.8.1 Exporting

101

3.8.2 Printing

102

Data analysis

103

3.9 Introduction

105

3.9.3 Fit points

106

-1/slope

107

3.11 Analysis method: None

111

3.11.2 PrimeQ Report

112

3.13.1 Assay requirements

114

3.13.2 Setup

114

3.13.3 Viewing the results

116

3.13.4 PrimeQ Report

118

3.14.1 Assay requirements

119

3.14.2 Setup

119

Crossing line

121

Noise threshold

121

3.14.3 Viewing the results

123

3.14.4 PrimeQ Report

124

3.14.5 Using Cq values

124

R² = 0.996 E = 1.977

126

Dissociation curve

134

3.15.1 Assay requirements

135

3.15.2 Setup

135

3.15.3 Viewing the results

141

3.15.4 PrimeQ Report

142

3.16.1 Assay requirements

144

3.16.2 Setup

144

3.16.3 Viewing the results

146

The plus-minus graph

147

Results table

147

3.16.4 PrimeQ Report

148

3.17.1 Assay requirements

150

3.17.2 Setup

150

3.17.3 Viewing the results

152

Reporter Selection

153

3.17.4 PrimeQ Report

155

3.18.1 Assay requirements

157

3.18.2 Setup

157

3.18.3 Viewing the results

158

Multi-read graph

159

3.18.4 PrimeQ Report

160

3.19.1 Multiplex setup

162

4 Technical information

165

4.1.1 Cleaning PrimeQ

167

4.1.2 Fuses

167

4.1.3 Insulation Testing

168

4.1.4 Mantenimiento

168

4.2.1 LED

169

4.2.2 Filters

169

4.2.3 Replacing the fuses

169

4.5 Accessories

170

4.6 Replacement parts

171

4.8 Packaging

174

4.9 Trademarks

174

5 Troubleshooting

175

5.1 Troubleshooting

177

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