Techne 3PrimeG User Manual

3Prime, 3PrimeX and 3PrimeG OPERATOR’S MANUAL Version 1.3 11/2012

9SICHERHEITS- INFORMATIONEN Lesen Sie diese Anleitung vor Verwendung des 3Prime bitte sorgfältig durch. ACHTUNG HOHE TEMPERATUREN STELLEN EINE GE

10 Ersatzkabel Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1 mm2 Adernquerschnitt und Europaste

11INFORMAZIONI DI SICUREZZA Leggere attentamente il presente manuale prima di usare il 3Prime. AVVERTENZA Le ALTE TEMPERATURE SONO PERICOLOSE in

12 7. Accendere lo strumento per mezzo dell'interruttore situato sulla parte retro dell'unità. Cavo di ricambio Qualora occorra sostitu

13INFORMACIÓN DE SEGURIDAD Lea atentamente este manual antes de utilizar el 3Prime. ADVERTENCIA LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que pueden

14 7. Encienda el instrumento usando el interruptor situado en la parte posterior de la unidad. Cable de repuesto Si es necesario sustituir el

15CONTACT INFORMATION For technical, sales or servicing information, contact your local Techne dealer or: UK Bibby Scientific Ltd. Beacon Road St

16 3PRIME SPECIFICATION 3Prime 3PrimeX 3PrimeG Sample capacity: 0.2ml 24 48 48 Sample capacity: 0.5ml 18 30 30 Block temperature range 4ºC (

17INSTALLATION See also the Safety Information sections on pages 5 to 14. Front view Rear view 1. Lid adjustment knob (3PrimeX

18 6. With the 3PrimeX and G, the heated lid has a rotating knob to adjust the lid pressure on the samples, allowing for a variety of consumables to

1CONTENTS PAGE INTRODUCTION 3 BEFORE USE 4 Unpacking 4 SAFETY INFORMATION 5 English 5 Français 7 Deutsch 9 Italiano 11 Español 13 CONTACT IN

19GENERAL SOFTWARE FEATURES INTRODUCTION The 3Prime software allows the user to quickly and easily create thermal cycling programs using

20 INCREMENTED/DECREMENTED TIME AND TEMPERATURE The software allows the user to either increment or decrement the time and/or temperature of a step

21The Tm is defined as the temperature at which half the DNA strands are single stranded and half are double stranded when base paired to a complimen

22 PROGRAMMING TEXT, TEMPERATURE AND TIME This section gives instructions for the basic programming of text, temperature and time whic

23Simple temperature entry • If necessary, first touch the button next to the parameter (e.g. Heated Lid) to turn it ON. • Next touch the Temp bu

24 Advanced temperature entry • First ensure the number of cycles for the stage has been set then enter the required hold temperatu

25The temperature value will now appear orange in the programming screen indicating that it contains a modified function. Note: It is not possible t

26 The temperature value will now appear orange in the programming screen indicating that it contains a modified function. Note: It is not possible

27• Enter the time using the up and down arrow keys (h, m, s). • Once the time is correct, touch OK to return to the previous screen. Thi

28 A new screen will open which is similar to the simple time entry screen. • Enter the required value (h, m, s) followed by OK. • If an entered

2FAQs 49 TECHNICAL SUPPORT AND SERVICING 50 ADDITIONAL INFORMATION 51 User Maintenance 51 Fault Finding 51 Fuses 51 Insulation Testing 51 ACCESSO

29INSTRUMENT SETTINGS AND DEFAULTS Touch the Settings button to access the instrument Settings menu. • Touch any of the buttons to view and edit t

30 If Auto Resume is set to Always and the power is interrupted, on return of the power supply, a message will be displayed on the screen giving deta

31• Touch Back to return to the Instrument Settings main menu. • Touch More Settings for further Instrument Settings. Administrator Settings This

32 Note: It is recommended that the administrator keeps a record of the new password. • Touch OK to return to the Administrator menu or Cancel to r

33Pause This will cause the unit to pause before the program starts at the ambient temperature of the block (unless Sample Cooling has been set to O

34 SERVICE SETTINGS The service settings are for the use of authorised service engineers only. A service engineer’s password is required to access

35• Touch the A C G T buttons to enter the primer sequence. • Touch the arrow to delete the last character. • Use the < and > keys to scro

36 CREATE A NEW PROGRAM From the Home Screen, touch the New Program button. This will open a screen where you can build a program by adding stages a

37Ramp rate • Touch the ramp rate setting to adjust the ramp rate. The range is 0.1ºC/s (minimum) to 3.0ºC/s maximum for heating and 2.1ºC/s for co

38 THE MORE BUTTON The More button can be used to access the Program Options and Instrument Settings defaults for editing in the current program.

3INTRODUCTION The 3Prime range is a family of personal thermal cyclers designed to accurately control the temperatures of samples betw

39 Initial denaturation, Final Extension and Final Hold • Touch the time and temperature buttons to edit these if required. • Use the Down arrow

40 SETTINGS • Touch the Settings button to view the instrument defaults as set up in the instrument Settings module. Any of these parameters can

41RUN A PROGRAM From the Home Screen, touch the Run button. This will open a screen where you can select a program to run, edit, copy or delete.

42 RUN Touching the Run button sends the selected program to the 3Prime, ready to start the run. The Pre-run screen This screen shows a step-by s

43• Touch Temperature log to view the block temperatures. The previous two minutes of temperature readings can be viewed by scrolling across the scr

44 Editing password protected programs If the edited program is password protected, touching Save will open the message box shown opposite. To save t

45To copy a program file to or from a USB memory stick: • Insert a USB memory stick into the port on the front of the unit. A message screen will

46 SEARCH Programs saved on the unit can be searched by program name. • Touch Search followed by the button on the Search screen. • Ent

47UPDATING THE 3PRIME SOFTWARE The 3Prime software can be updated by downloading the latest version from the Techne website www.techne.

48 UPGRADING FROM 3PRIMEX TO 3PRIMEG The 3PrimeX thermal cycler can be easily and quickly upgraded to the gradient 3PrimeG version by purchasing th

4BEFORE USE Before using the 3Prime please ensure you have read this manual carefully. If there is any doubt relating to the proper us

49FAQS Q1 How do I adjust the pressure of the heated lid for my tubes or plates? A1 For thermal cyclers with lid adjustment knobs, rotate the knob a

50 TECHNICAL SUPPORT AND SERVICING If you require further technical or application assistance please contact Techne at: E-mail: technehelp@bibby-sc

51ADDITIONAL INFORMATION USER MAINTENANCE Before cleaning your unit, disconnect it from the power supply. The outer case of the 3Prime may be cleane

52 ACCESSORIES The following accessories can be obtained from Bibby Scientific Ltd. or your Techne distributor: Product code Description 3PRIMEX/US

53

5SAFETY INFORMATION Please read all the information in this manual before using the 3Prime. WARNING HIGH TEMPERATURES ARE DANGEROUS: they can cause s

6ENVIRONMENTAL CONDITIONS The 3Prime is designed operate under the following conditions: • Indoor use • Ambient temperature range +5ºC to +40ºC •

7L'INFORMATION DE SÛRETÉ Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le 3Prime. AVERTISSEMENT Les TEMPÉ

8Câble de rechange S’il s’avère nécessaire de remplacer le cordon d’alimentation, utiliser un câble de 1 mm² conforme à la norme H05VV-F relié à une