Techne TC-PLUS User Manual

TC-PLUS OPERATOR’S MANUAL VERSION 1.7 11/2012 Part number LTC1038

8Câble de rechange S’il s’avère nécessaire de remplacer le cordon d’alimentation, utiliser un câble de 1 mm² conforme à la norme H05VV-F relié à une

9SICHERHEITS- INFORMATIONEN Lesen Sie diese Anleitung vor Verwendung des TC-PLUS bitte sorgfältig durch. ACHTUNG HOHE TEMPERATUREN STELLEN EINE G

10 Ersatzkabel Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1 mm2 Adernquerschnitt und Europaste

11 INFORMAZIONI DI SICUREZZA Leggere attentamente il presente manuale prima di usare il TC-PLUS. AVVERTENZA Le ALTE TEMPERATURE SONO PERICOLOSE

12 7. Accendere lo strumento per mezzo dell'interruttore situato sulla parte anteriore dell'unità. Cavo di ricambio Qualora occorra sos

13 INFORMACIÓN DE SEGURIDAD Lea atentamente este manual antes de utilizar el TC-PLUS. ADVERTENCIA LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que puede

14 Cable de repuesto Si es necesario sustituir el cable de alimentación, se debe utilizar un cable de 1mm2 de código armonizado H05V

15 CONTACT INFORMATION For technical, sales or servicing information, contact your local Techne dealer or: UK Bibby Scientific Ltd. Beacon Road, S

16 TC-PLUS SPECIFICATION TEMPERATURE Block temperature range 4ºC (or 20ºC below ambient) to 100ºC Block uniformity <±0.3ºC Temperature accuracy

17 INTERCONNECTIVITY Connection to PC control software Yes (Satellite units only) Software updates Free of charge from www.techne.com COMMUNICAT

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18 INSTALLATION See also the Safety Information sections on pages 5 to 14. Front view TC-PLUS TC-PLUS Satellite 1. TC

19 Installation 1. Place the unit on a suitable flat bench, ensuring that the air inlet vents on the front and rear are free from obstruction. Two u

20 GENERAL PROGRAMMING FEATURES INTRODUCTION The TC-PLUS software allows the user to quickly and easily create thermal cycling programs using a simp

21 The Ice Bucket and Ligation programs are simple temperature holds, with the former having an infinite hold at 4ºC and the latter at 15ºC. In both

22 OLIGONUCLEOTIDE TM CALCULATOR One of the most important parameters in a PCR is the annealing temperature of the reaction. This can be determined

23 TEMPERATURE ENTRY When touching a temperature parameter a temperature entry screen will open. There are two forms of temperature entry screen:

24 A new screen will open which is similar to the simple temperature entry screen. The maximum increase in temperature per cycle (based on the number

25 A new screen will open which is similar to the simple temperature entry screen. The maximum decrease in temperature per cycle (based on the number

26 A new screen will open which is similar to the simple temperature entry screen. The maximum temperature gradient (based on the set hold temperatur

27 • Touch Cancel to return to the previous screen if no changes are required. Advanced time entry • First ensure the number of cycles for the st

1CONTENTS PAGE INTRODUCTION 3 BEFORE USE 4 Unpacking 4 SAFETY INFORMATION 5 English 5 Français 7 Deutsch 9 Italiano 11 Español 13 CONTACT IN

28 The time value will now appear orange in the programming screen indicating that it contains a modified function. To decrease the step

29 The time value will now appear orange in the programming screen indicating that it contains a modified function. Note: It is not possible to bot

30 INSTRUMENT SETTINGS AND DEFAULTS Touch the Settings button to access the instrument Settings menu. • Touch any of the buttons to view and edit

31 INSTRUMENT SETTINGS Temperature Log Recording To record the temperature logs (block temperature profile) of each run, touch the button to set th

32 Time • Touch Time in order to enter the current time using the 24 hour clock. • Use the + and – keys to set the time values. • When the corre

33 Remove Passwords • From the Administrator menu touch Remove Passwords. • Touch the file with the password to be removed. • Touch Remove Passw

34 PROGRAM DEFAULTS The program default settings will apply to every new program created on the TC-PLUS. However individual programs can be edited

35 temperature. • Touch More Settings for further program defaults or Back to return to the Settings menu. Final Extension Use this to add a final

36 OLIGO TM CALCULATOR The oligo Tm calculator can be used to estimate the Tm of the PCR primers and help in selecting an appropriate annealing tem

37 • To check the Tm of another primer with the same primer and salt concentrations, touch Back and enter the new primer sequence. • Touch Reset

2Searching for a program not in the shortcut list 49 Running a program not on the shortcut list 51 EDITING A PROGRAM 53 Editing saved programs 53

38 CREATE A NEW PROGRAM From the Home Screen, touch the Create A New Program button. This will open a screen where you can build a program by adding

39 INSERT/DELETE A STAGE A stage is a section of a program that consists of a number of individual steps that can be cycled (repeated) a defined num

40 INSERT/DELETE A STEP Insert a new step • Touch Insert a new step to insert the next step in the current stage. • Edit the new step temperatur

41 User User names can be entered and linked to the program to help in searching and identifying programs. • To enter a user name, touch the None

42 Keywords Keywords can be entered and linked to the program to help in searching for related programs. • To enter a keyword, touch the None sel

43 Passwords A password can be created to protect the program from being edited and deleted by other users. The password will be requested if a user

44 SETTINGS • Touch the Settings button to view the instrument defaults as set up in the instrument Settings module. Any of these parameters can

45 DELETE This is used to delete an entry. First touch the required segment followed by Delete. UP AND DOWN ARROWS Use these buttons to scroll t

46 RUN A PROGRAM From the Home Screen, touch one of the shortcuts to run one of the listed programs, or touch Run a Program to search and select fro

47 Note: If the user attempts to send a program to unit while it is already running, the warning screen shown opposite will be displayed. • Touch O

3INTRODUCTION The TC-PLUS is an advanced gradient thermal cycler designed to accurately and rapidly control the temperatures of samples between 0ºC

48 THE RUN SCREEN The Run Screen will be displayed after a program has been started. It shows the instrument status and the progress of a run. Onc

49 Once the run has finished, Program Finished will be displayed instead of End time. Viewing a running program From the Home Screen: • Touch Curr

50 Search the programs by user • Touch Search by User and this will open the list of user names stored on the unit. • Select the required user nam

51 RUNNING A PROGRAM NOT ON THE SHORTCUT LIST • Once the required program has been identified, touch it to highlight it. The Program Selector Scre

52 Note: If the user attempts to send a program to unit while it is already running, the warning screen shown opposite will be displayed. • Touch O

53 EDITING A PROGRAM EDITING SAVED PROGRAMS First select the required program by searching the Run a program module. • To edit a selected progra

54 EDITING PASSWORD PROTECTED PROGRAMS If the edited program is password protected, touching Save will open the message box shown opposite. To save

55 CURRENT STATUS The Current Status area of the Home Screen provides status information on the TC-PLUS and any connected Satellite units. On the H

56 VIEW FILES The View files module opens the File Manager and allows access to all the program and temperature log files. From here it is possible

57 Search the files by user • Touch Search By User and this will open the list of user names stored on the unit. • Select the required user name b

4BEFORE USE Before using the TC-PLUS please make sure you have read this manual carefully. If there is any doubt relating to the proper

58 VIEWING A PROGRAM FILE • Touch Programs And Logs to display Only Programs. • Search for the required program file and touch it to highlight i

59 When opening a temperature log file, the Temperature Log Monitor screen will be displayed. To view the complete temperature log, drag the mouse ac

60 To copy files to a USB memory stick: • Insert a USB memory stick into the port on the front of the unit. A message screen will appear confirming

61 DELETING FILES Unused or old files can be permanently deleted from the unit or a USB memory stick. • Select one or more files for deleting by

62 UPDATING THE TC-PLUS SOFTWARE The TC-PLUS software can be updated by downloading the latest version from the Techne website www.techne.com. You w

63 REMOVING AND EXCHANGING BLOCKS The thermal cycling block can be removed and exchanged for one of a different format or for detailed cleaning. •

64 FAQS Q1 How do I adjust the pressure of the heated lid for my tubes or plates? A1 No adjustment is necessary. The lid automatically applies the c

65 TECHNICAL SUPPORT AND SERVICING If you require further technical or application assistance please contact Techne at: E-mail: technehelp@bibby-sc

66 ADDITIONAL INFORMATION USER MAINTENANCE Before cleaning your unit, disconnect it from the power supply. The outer case of the TC-PLUS may be clea

67 ACCESSORIES The following consumables and accessories can be obtained from Bibby Scientific Ltd. Or your Techne distributor: Product

5SAFETY INFORMATION Please read all the information in this manual before using the TC-PLUS. WARNING HIGH TEMPERATURES ARE DANGEROUS: they can cause

68 REPLACEMENT PARTS The following replacement parts can be obtained from Bibby Scientific Ltd. or your Techne dealer: Product code Description HH1

6ENVIRONMENTAL CONDITIONS The TC-PLUS is designed operate under the following conditions: • Indoor use • Ambient temperature range +5ºC to +40ºC •

7L'INFORMATION DE SÛRETÉ Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le TC-PLUS. AVERTISSEMENT Les TEMP