Techne PrimeG User Manual Page 17

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PRIME SPECIFICATION
TEMPERATURE
Block temperature range 4ºC (10°C during cycling) to 100ºC
Block uniformity (at 55ºC) 0.3ºC
Temperature accuracy (at 55ºC) 0.25ºC
Gradient availability (PrimeG only) Between 30ºC and 80ºC
Maximum gradient (PrimeG only) 29ºC (25°C Combi-Block; 19ºC 384-well block)
Minimum gradient (PrimeG only) 1ºC
Column uniformity with a 10ºC gradient
(PrimeG only)
0.3ºC
Pre-run sample cooling Yes, 4ºC
Temperature set point adjustment 0.1ºC
HEATING/COOLING RATE
Maximum heating rate 3.4ºC/s
Minimum heating rate 0.1ºC/s
Maximum cooling rate 1.0ºC/s
Minimum cooling rate 0.1ºC/s
HEATED LID
Selectable heated lid temperature 35 to 115ºC or off
Lid uniformity ±5ºC
Pre-heat lid Yes
Warm up time from ambient <2 min
Over-temperature cut-out Yes
Regulated heated lid pressure Manually adjustable
The heated lid is only operational if the block temperature is set above 35ºC
PROGRAMMING
Program interface 5.7” colour VGA LCD touch screen
Maximum number of programs stored 1000
Maximum number of stages per program 10
Maximum number of steps 10
Maximum number of cycles per stage 99
Programmable ramp rate Yes, 0.1ºC/s steps
Maximum hold time 4h 59m 59s
Minimum hold time 1s
Gradient calculator (PrimeG only) Yes
Incremented/decremented temperature Yes
Incremented/decremented time Yes
Pause facility Yes
Program naming Alpha numeric plus symbols
Password protection Yes
Keyword selection Yes
Run completion time Yes
End of program alarm Yes
Auto resume on power failure Yes, always or never
Oligonucleotide T
m
calculator Yes, based on the Nearest-Neighbour method
1
Software updates Free of charge from www.techne.com
1
Breslauer, K.J.; Frank, R.; Blocker, H. and Marky, L.A. (1986) Proc. Natl. Acad. Sci. USA 83, pp
3746-3750.
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