Techne TC-5000 User Manual

TC-5000OPERATOR'S MANUALIssue 15/09

L'appareil a été testé en matière de perturbations radioélectriques et est certifié selon la normeEN61326.GARANTIEL'appareil est garanti con

SICHERHEITS - UND INSTALLATIONSINFORMATIONENSICHERHEIT DES BEDIENPERSONALSLesen Sie diese Anleitung vor Verwendung des Thermocyclers bitte sorgfältig

1. Alle Geräte von Bibby Scientific Ltd werden mit einem Netzkabel geliefert, das entweder eingestecktwird oder fest mit dem Gerät verbunden ist.2. Vo

Hinweis: Die Gerätespezifikationen beziehen sich auf eine Umgebungstemperatur von 20°C undkönnen sich außerhalb des Bereichs 10°C bis 30°C verschlecht

INFORMACIÓN DE SEGURIDAD E INSTALACIÓNSEGURIDAD DEL OPERARIOLea atentamente este manual antes de utilizar el ciclador térmico. Si el equipo no se util

2. Antes de conectar el instrumento al suministro eléctrico, compruebe que el voltaje coincida con elindicado en la placa de régimen (situada en la pa

especificaciones pueden empeorar si se utiliza el instrumento fuera del intervalo de temperaturacomprendido entre 10°C y 30°C.Se han realizado pruebas

INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONESICUREZZA DELL'OPERATORELeggere attentamente il presente manuale prima di usare il ciclatore t

2. Prima di collegare lo strumento all'alimentazione elettrica di rete, controllare la tensioneconfrontandola con la targhetta riportante i valor

Nota: le specifiche di controllo sono indicate ad una temperatura ambiente di 20°C. Le specifichepotrebbero peggiorare fuori da una temperatura ambien

CONTENTSPAGEINTRODUCTION 3SAFETY AND INSTALLATION INFORMATION 4Operator Safety 4Warning 4Unpacking 4Installation 4Replacement Cable 5Working Condition

19INSTRUMENT CONNECTIONSRS232/RS485Selector: Select RS485for multiple units orRS232 for a singleunitConnection Switch:Select “Daisy Chain”for all unit

20CONTACT INFORMATIONFor technical, sales or servicing information, contact your local Bibby Scientific Ltd dealer or:UK and Rest of WorldBibby Scient

TC-5000 SPECIFICATIONTemperatureGradient Range 20°C to 70°CMaximum Gradient 30°CMinimum Gradient 1°CNumber of temperature probes 4Block temperature ra

MiscellaneousHeight x Width x Length 260mm x 220mm x 420mmWeight 12.5kgPower consumption 620W (including the heated lid)Parallel port YesSerial port R

23TC-5000 OPERATION AND MAINTENANCEThe TC-5000 has many features which increase the flexibility of the instrument and improve the ease ofuse. The foll

24It is important to remember that the greater the gradient, the poorer the uniformity across the block,therefore fine-tuning may be required to estab

25A heated lid mat (Part No. 6104978) is supplied with the TC-5000; this is for use with 96-well PCRplates sealed with an adhesive seal. The rubber ma

26PC CONNECTIONSIt is possible to connect either a single thermal cycler or a series of instruments to a computer. Theoptions are: 1. To connect a sin

27CLEANING THE TC-5000Prior to cleaning the thermal cycler it is important to disconnect the unit from the power supply and ifit has been in use allow

28REMOVING THE BLOCKThe thermal cycling block can be removed for detailed cleaning or replacement.First ensure that the unit is disconnected from the

2INSTRUMENT CONNECTIONS 19CONTACT INFORMATION 20TC-5000 SPECIFICATION 21TC-5000 OPERATION AND MAINTENANCE 23Gradient 23Adjustable Heated Lid 24Adjusta

29After the self tests have been completed thescreen opposite is shown unless the unit ispowered up by auto-restart. If there is aprogram running and

30To access the programming software press the"System User" button on the start-up screen.The System User menu will then appear.The options

31Parameter 2 Step PCR 3 Step PCR RT PCRHeated Lid 105°C 105°C 105°CHeated lid before program On On OnPause before program Off Off OffInitial denatur

32OPTION 1: COPYING AN EXISTINGTEMPLATETo access the program templates select"Programs" in the System Users menu. The cursor arrows can be

Select the copied program.The program cannot be changed at this pointso press "Edit" to make any changesrequired.The program can be copied,

34OPTION 2: EDITING AND SAVING ANEXISTING TEMPLATESelect the template from the program menu.Select "Edit"All of the parameters can be edited

35OPTION 3: CREATING A NEW PROGRAMThe final option is to create a completely newprogram by selecting "New" in the SelectProgram menu. This s

The gradient on the TC-5000 can be set from 1 to 30°C across the temperature range 20 to 70°C.Outside this range the accuracy of the different columns

37The time can be set in hours (h), minutes (m) orseconds (s). Type in the time required followedby the unit. Use the to move across thescreen to

38The Hold Temperature of the incremented/decremented step is the defined Hold Temperature plus thesummation of the increments/decrements. The first c

INTRODUCTIONThe TC-5000 is a gradient thermal cycler, designed to accurately control the temperatures of samplesbetween 4°C and 99°C. It has many scie

39INSERTING A PAUSESelecting "Pause" adds a stop to theprogram, which when running requires userintervention before the program cancontinue.

40Select the program required, using the cursorkeys to view all the available programs.Press "Run".The run screen is then displayed; if the

41As the program continues the temperaturesare displayed on the screen, as shownopposite.This screen is displayed when the run iscomplete. If there is

42This screen is displayed if there is a power lossduring the running of the program and theauto-restart function is switched on. Thedisplay shows the

43Initial denaturation: Many experimentalprotocols require a high temperatureincubation to remove any secondary structureof the nucleic acid template.

44GRADIENT CALCULATORThe gradient is set within the program (see page 36 for further details), however, it is not possible toview the actual temperatu

45SYSTEM ADMINISTRATIONThis section of the software contains settings for the instrument, rather than the actual programs.From various sub-menus it is

Program Passwords: Enables an administrator to change the password for any program.Sys Admin Password: Allows the administrator to change the administ

47SYSTEM TESTThis is a diagnostic test to ensure that theReal Time Clock settings are correct.CONTROLLER RESETIf "Confirm Reset" is pressed

48SYSTEM CONFIGURATIONThis contains all the settings for programming the instrument such as units of temperature, dateformat, language, and date and t

SAFETY AND INSTALLATIONOPERATOR SAFETYPlease read this manual carefully before using the thermal cycler. If the equipment is not used in themanner des

49Date: The date can be corrected by the user by using the numerical interface shown above.Time: The time can be corrected by the user by using the nu

50FREQUENTLY ASKED QUESTIONSQ1 What is "Hot start"?A1 The Hot Start step is used to pause the machine at a specific temperature, typically a

51ADDITIONAL INFORMATIONBrief fault finding notes and a list of replacement parts are given in this section.Note: that this equipment should only be d

52Product Number Description QuantityFHSEALSD Heat sealer 1FHSPA96 Heat sealer plate adapter for 96-well plates 1FHSPA384 Heat sealer plate adapter fo

Bibby Scientific LtdBeacon Road, Stone, Staffordshire, ST15 0SAUnited KingdomTel: +44 (0)1785 812121 Fax: +44 (0)1785 813748 email: techne@bibby-

rating plate (located on the back of the unit). Ensure that the voltage selector switch (located abovethe On/Off switch) is set to the appropriate vol

6GUARANTEEThe instrument is guaranteed against any defect in material or workmanship for the period specifiedon the enclosed guarantee card. This peri

SÉCURITÉ ET CONSIGNES D'INSTALLATIONSÉCURITÉ DE L’OPÉRATEURLire attentivement le présent manuel avant d'utiliser le cycleur thermique. Si ce

2. Avant de raccorder l'appareil à l'alimentation électrique sur secteur, vérifier la tension par rapport àla plaque signalétique (située au