Techne PrimeQ User Manual download pdf (Page 37)

1.18 LED power settings

The LED light source has variable power settings. This prevents PMT blinding and brings

fluorescence into its linear range for certain applications. The role of this option is to cut down

excess light from the LED source in high fluorescence applications. The three power options are:

Low (50mA), Medium (100mA), and High (200mA).

The power level is specified by the user when programming the PCR in the application software

(at the same time as specifying the fluorophore filter); the default setting is

Medium

. The three set

power levels are not user-adjustable.

1.19 Inserting a plate

•

To insert a plate, push the orange panel to release the latch, and open the drawer by sliding

it towards you.

•

To close the drawer push it home until the latch clicks and the drawer stays shut.

1.19.1 Inserting a previously run plate

Users should note that when the thermal cycler plates are heated, the plastic may tend to distort

slightly. This means that if the plate is removed from the block then either returned or transferred

to another block, the fit can be poor. If returning or transferring a plate is unavoidable then the

following procedure is recommended to improve the fit:

•

If a heat seal has been used, place the plate in the heat-sealer and seal the plate for 3-4

seconds; rotate the plate 180 degrees; seal the plate for 3-4 seconds.

•

Insert the plate in the block and close the drawer.

1.19.2 Running a plate

Perform the loading procedure on the prepared plate following the instruction given when the

experimental run is started in the Quansoft operating software.

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Techne PrimeQ User Manual Page 37