Techne PrimeQ User Manual Page 59
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- Table of contents
- TROUBLESHOOTING
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59
View:
Allows the user to change the way the files appear
in the file view. Choose to display simply as icons or
display with further details such as file name and size.
Tools:
Provides direct access into the editor of choice -
simply click on the editor name. The
Change Quansoft
folder…
command allows the user to change the
destination of any saved files. Quansoft will automatically
save all files to My Documents\Quansoft; change the
destination here if required.
Help:
Off- and on-line resources. Click
Quansoft Help
(short cut key F1) for access to this Operator guide, or click
on
Techne on the Web
to link to the Techne website.
Clicking on
About Quansoft
will bring up details about the
software including the version and serial number.
Registered users of Quansoft will be kept informed of
software updates.
3.3.6 Accessing the editors
The user can access the Quansoft editors by the following routes:
•
Home page:
Click the round shortcut icons as discussed in section 3.3.2.
•
Menu bar:
Go to
Tools
and click on the editor of choice.
•
Library files:
Clicking on a library file will open up that file within the relevant editor. Either
browse a library file from the Home page or browse from within an editor.
•
New or Browse
: Clicking on these buttons in the program or plate layout pane of the
Experiment or Results Editor opens up the relevant editor within. Files can then be browsed,
edited or created from new.
•
Start menu:
Click on the Windows
Start
button in the bottom left hand corner. Choose
All
Programs/Techne/Quansoft/Editors
and then select the editor of choice.
- OPERATOR’S MANUAL 1
- How to use this guide 3
- Contents 7
- 1 Introduction 13
- 1.1 PrimeQ 15
- 1.2 Quansoft 15
- 1.2.3 Program Editor 16
- 1.2.4 PrimeQ reports 16
- 1.2.5 Data Analysis 16
- 1.3 Unpacking 17
- 1.4 Warning! 17
- 1.5 Uses of PrimeQ 17
- 1.6 Plates 17
- 1.7 Thermal seal 18
- 1.8 Contacting us 18
- 1.9 Installing the hardware 18
- 1.9.3 Operator safety 20
- 1.9.4 Installation 20
- 1.10 Before switching on 23
- 1.11 Technical Specification 24
- 1.12 Working conditions 25
- 1.13 Guarantee 25
- WARRANTY 26
- IF BROKEN 26
- 1.14.4 Software upgrades 29
- 1.15.1 Function keys 30
- 1.15.2 Power up screen 30
- 1.15.3 Beeper 30
- 1.15.4 Before you start 31
- 1.15.5 Start-up procedure 31
- 1.16.8 Points to remember 35
- 1.18 LED power settings 37
- 1.19 Inserting a plate 37
- 1.20 After use 38
- 2 Getting started 39
- 2.1 Introduction to PrimeQ 41
- 2.2.1 PCR 42
- 2.3.1 Intercalating dyes 44
- 2.3.3 Molecular Beacons 45
- 2.4 System overview 46
- 2.5 Optics module 46
- 2.6 Thermal cycling module 47
- 2.7 Scanning module 48
- 3 Quansoft 49
- 3.1 Introducing Quansoft 51
- 3.2 Software overview 52
- 3.2.1 Home page 53
- 3.2.2 The Editors 53
- 3.2.2.3 Program Editor 54
- 3.2.2.4 Results Editor 54
- 3.3 Using Quansoft 55
- 3.3.2 Main screen 56
- 3.3.3 Navigation bar 56
- 3.3.4 Title bar functions 57
- 3.3.5 Menu bar functions 58
- 3.3.6 Accessing the editors 59
- 3.4.1 An Overview 60
- 3.4.3 Setting up a program 62
- Comment [MO1]: 65
- Discussed between 65
- Matt and Christian 65
- 3.5 Running an experiment 86
- 3.5.2 Monitoring the run 89
- 3.6 LED intensity settings 92
- 3.7 Results Editor 93
- 3.7.3 Editing a graph 96
- 3.7.5 Log/Audit trail 99
- 3.7.6 Report 99
- 3.8.1 Exporting 101
- 3.8.2 Printing 102
- Data analysis 103
- 3.9 Introduction 105
- 3.9.3 Fit points 106
- -1/slope 107
- 3.11 Analysis method: None 111
- 3.11.2 PrimeQ Report 112
- 3.13.1 Assay requirements 114
- 3.13.2 Setup 114
- 3.13.3 Viewing the results 116
- 3.13.4 PrimeQ Report 118
- 3.14.1 Assay requirements 119
- 3.14.2 Setup 119
- Crossing line 121
- Noise threshold 121
- 3.14.3 Viewing the results 123
- 3.14.4 PrimeQ Report 124
- 3.14.5 Using Cq values 124
- R² = 0.996 E = 1.977 126
- Dissociation curve 134
- 3.15.1 Assay requirements 135
- 3.15.2 Setup 135
- 3.15.3 Viewing the results 141
- 3.15.4 PrimeQ Report 142
- 3.16.1 Assay requirements 144
- 3.16.2 Setup 144
- 3.16.3 Viewing the results 146
- The plus-minus graph 147
- Results table 147
- 3.16.4 PrimeQ Report 148
- 3.17.1 Assay requirements 150
- 3.17.2 Setup 150
- 3.17.3 Viewing the results 152
- Reporter Selection 153
- 3.17.4 PrimeQ Report 155
- 3.18.1 Assay requirements 157
- 3.18.2 Setup 157
- 3.18.3 Viewing the results 158
- Multi-read graph 159
- 3.18.4 PrimeQ Report 160
- 3.19.1 Multiplex setup 162
- 4 Technical information 165
- 4.1.1 Cleaning PrimeQ 167
- 4.1.2 Fuses 167
- 4.1.3 Insulation Testing 168
- 4.1.4 Mantenimiento 168
- 4.2.1 LED 169
- 4.2.2 Filters 169
- 4.2.3 Replacing the fuses 169
- 4.5 Accessories 170
- 4.6 Replacement parts 171
- 4.8 Packaging 174
- 4.9 Trademarks 174
- 5 Troubleshooting 175
- 5.1 Troubleshooting 177
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