Techne PrimeQ User Manual download pdf (Page 46)

2.4 System overview

PrimeQ is a real-time PCR unit into which the operator places a 96-well plate containing the

samples to be analysed. The unit is connected to a PC via the USB port on which the application

software (Quansoft) is run. The software allows the user to:

Create

a PCR program defining dyes, read points, thermal cycling and analysis methods.

Run

the program.

Analyse

the fluorescence readings.

•

Thermal cycler:

The specialized block cycles the temperature according to the pre-

defined PCR program.

•

Reader:

Allows fluorescence within each well of the plate to be read at

specified points during the PCR program. The reader excites the

samples at one wavelength and then detects the emitted fluorescence

at another.

•

Software:

The unit requires a PC installed with the application software,

Quansoft, for protocols to be defined and downloaded and for the

data to then be analysed.

•

Consumable:

The unit accommodates a low-profile 96-well plate (skirted or non-

skirted) sealed with optical sealing film.

PrimeQ consists of three key parts: the optics module; a thermal cycler module and a scanning

module.

2.5 Optics module

The role of the optics module is to deliver excitation light into each well and measure the emitted

light at defined points in the PCR cycle. The light source is a solid state white light source with the

excitation and emission wavelengths controlled by a system of filters and a dichroic mirror housed

in the user-changeable filer cartridges. Four such filter cartridges can be accommodated at any

one time in the filter carousel.

1 2 ... 41 42 43 44 45 46 47 48 49 50 51 ... 180 181

No comments

Techne PrimeQ User Manual Page 46